Device

Part:BBa_K4611021:Design

Designed by: Saiyu Luo   Group: iGEM23_CJUH-JLU-China   (2023-10-09)


His-SUMO-LwaCas13a under T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3222
    Illegal PstI site found at 3243
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3222
    Illegal PstI site found at 3243
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3222
    Illegal BglII site found at 202
    Illegal BglII site found at 1678
    Illegal BglII site found at 1888
    Illegal BglII site found at 2155
    Illegal BglII site found at 2920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3222
    Illegal PstI site found at 3243
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3222
    Illegal PstI site found at 3243
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The sequence of LwaCas13a is from Leptotrichia wadeii genome, but is human codon optimized. Original gene is from Genbank:Leptotrichia wadei F0279. Provided by pC013-Twinstrep-SUMO-huLwCas13a(addgene)

References